This work followed earlier approaches to the application of the Van Slyke (3) method of protein precipitation and the Lowry method of protein determination to evaluation of purified virus preparations that were carried out in the Lilly Laboratories by Dr. John Ross. Determination of Total Protein by the Lowry Method Using the BioTek Instruments’ ELx808 Microplate Reader It’s Fast, It’s Easy, and It Turns Blue Quantitation of total protein content of samples is a measurement common to many applications in basic science and clinical research. ScienceDirect ® is a registered trademark of Elsevier B.V. ScienceDirect ® is a registered trademark of Elsevier B.V. The total protein concentration is exhibited by a color change of the sample solution in proportion to protein concentration, which can then be measured using colorimetric techniques. Rose and for very competent technical assistance to Mr. Steven Mundy of these laboratories. It is named for the biochemist Oliver H. Lowry who developed the reagent in the 1940s. Precision among replicates was good; the greatest difference of a single value from the average was 1.7%. Copyright © 2020 Elsevier B.V. or its licensors or contributors.
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For some time it was the method of choice for accurate protein determination for cell fractions, chromatography fractions, enzyme preparations, and so on.
This work followed earlier approaches to the application of the Van Slyke (3) method of protein precipitation and the Lowry method of protein determination to evaluation of purified virus preparations that were carried out in the Lilly Laboratories by Dr. John Ross.
Copyright © 1969 Wiley-Liss, Inc., A Wiley Company. Published by Elsevier Inc. All rights reserved. Quantitation of total protein content is a measurement common to many applications in basic science research and routine clinical laboratory practice.
Application of Lowry Protein Determination to Influenza Vaccine. He also thanks Dr. R. S. Baker, Biological Research Department of Eli Lilly and Company, for samples of influenza vaccine. Because of the tendency of the virus to aggregate, additional techniques such as sonoration were needed. In the biuret reaction, copper interacts with four nitrogen atoms of peptides to form a cuprous complex. The Blue color developed by the reduction of the Phosphomolybdic-Phosphotungastic components in the Folin-ciocalteau reagent by the amino acids Tyrosine and Tryptophan present in the protein plus the color developed by the biuret reaction of the protein with the color developed by the biuret reaction of the protein with the alkaline cupric tartrate is measured in the Lowry method. The Lowry protein test has been successfully applied to the determination of microgram amounts of protein in influenza vaccine.
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