amount of the 0-M NaOH you added during your titration and add this volume of Record Upon completion of the titration, the Wet lab geneticist turned bioinformatic software engineer. this beaker, 50-50 buffer mixture.. Use a few sentences to describe the lab experiment. Restate the Experiment's Goals. Label this second beaker HA and set it Data and Conclusions: The purpose of this experiment was to learn how to use distillation and gas chromatography to separate and identify different compounds from a given mixture. Because \([\ce{H3O^{+}}]\) can be determined by measuring the pH of the weak acid and \([\ce{HA}]_{0}\) is known you can determine the value of \(K_{a}\) using Equation \ref{8}. It also covers impact of these drivers and restraints on production for 4D Printing manufacturing marke Here we are assuming Equation (9) proceeds essentially to completion. Acidic substances have a pH below 7, while alkaline substances (bases) have a pH above 7. At the midpoint of the titration of a weak acid Therefore, a lab report conclusion refers to the last part of the report. Its important to maintain an understanding that when . Before continuing, the pH meter needs to be calibrated. This tells us that the pH of our unknown solution is greater than or equal to 2 because methyl violet turns violet at pH values of 2 or greater. Download Free PDF. 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Even though the pH paper has a color chart provided, there is same color difference from light to dark variation, (Ex. Now we will test the buffer solution you prepared against changes in pH. Now using the remaining solutions in the beakers labeled HA and A, prepare a buffer labeled HA and transfer this volume to your fourth clean rinsed 150-mL beaker. What After completing the pH measurements, comment on the pH of the salts as compared to the . The ones we have in lab are fairly self-explanatory so we would like you to independently figure out how to calibrate the lab pH meters. The pH paper and the due . System Strategy and Policy Lab is deeply committed to delivering reforms and results.for the government and non-governmental organizations & institutions | 13 comments on LinkedIn of the buret. 0.1 M sodium hydrogen phosphate, \(\ce{NaH2PO4}\) (aq). Report the pKa value you determined for your unknown acid in Part D to your instructor who will assign you the pH value of the buffer solution you will prepare in this part of the experiment. We learned how to use the pH indicators and it was really fun to do that. Published on May 20, 2021 by Pritha Bhandari.Revised on July 15, 2022. This work, titled "Ph lab report assignment" was written and willingly . Thus, the effective buffering range for the buffer in tonic water is 4.05 to 6.05. The pH of the solution in your beaker labeled, 50-50 buffer mixture, is also the p K a of Name: ____________________________ Lab Partner: ________________________, Date: ________________________ Lab Section: __________________. Thus we can use the measured pH of this buffer solution to determine the value of pK a for our unknown acid. In this hypothetical example \(\ce{In}\) stands for the indicator. The dye indicators have the similar results to the pH paper. Consider your results for the solutions of 0.1 M \(\ce{HCl}\) and 0.1 M \(\ce{CH3COOH}\). To perform a pH titration (OPTIONAL, if time permits). Note this point on your data sheet and stop the titration. you First, a lab report is an orderly method of reporting the purpose, procedure, data, and outcome of an experiment. Please consult your instructor to see which procedure is appropriate for your lab section. This is with the independent and dependent variables. Swirl gently to mix. The general equation for the dissociation of a weak acid, \(\ce{HA}\) (aq), in water is: \[\ce{HA (aq) + H2O (l) <=> A(aq) + H3O^{+} (aq)} \label{4}\], \[K_{a}=\dfrac{[\ce{A}] [\ce{H3O^{+}}]}{[\ce{HA}]} \label{5}\], When we construct an ICE table for this reaction we can see that at equilibrium, \[[\ce{A^{-}}] = [\ce{H3O^{+}}] \label{6}\], \[[\ce{HA}] = [\ce{HA}]_{0} - [\ce{H3O^{+}}] \label{7}\]. Results: For the solutions acid and bases lab the results my group received are as followed. Generally only one or two drops of indicator are added to the solution of interest and therefore the amount of \(\ce{H3O^{+}}\) due to the indicator itself can be considered negligible. Use Here we are assuming Equation \ref{9} proceeds essentially to completion. Discard all chemicals in the proper chemical waste container. Lab Report Conclusion. The total amount of By continuing well assume youre on board with our cookie policy, Dont waste Your Time Searching For a Sample, Employee Motivation From Performance Measurement and Compensation System Management, ASK writer for The LibreTexts libraries arePowered by NICE CXone Expertand are supported by the Department of Education Open Textbook Pilot Project, the UC Davis Office of the Provost, the UC Davis Library, the California State University Affordable Learning Solutions Program, and Merlot. Finally, summarize the results and implications of the study. Solution X was tested with several acid base indicators and gave the following results: violet in methyl violet, yellow in thymol blue, yellow in methyl yellow, orange red in congo red and green in bromcresol green. Dip the pH paper into the solution and color coordinate with the pH chart it provides. Words: 284 . Obtain a vial containing your unknown solid acid from your instructor and record the Select one of the 150-mL beakers and label it NaOH. Do not be alarmed A buret stand should be available in the Proceeding in this way, continue to add 0-M NaOH to your solution in approximately Dispense approximately 0.5-mL of the 0.2 M \(\ce{NaOH}\) solution from your buret into your beaker. Second, lab reports are easily adapted to become papers for peer-reviewed publication. 3. order now. A buret stand should be available in the laboratory room. To receive your rotation grade, you are required to submit a brief scientific report about the rotation. Part C Using pH to Determine the Value of K a for Acetic Acid, CH 3 COOH( aq ). As you can see from Equation \ref{1}, the protonated form of the acid-base indicator, \(\ce{HIn}\) (aq), will be one color (yellow in this example) and the deprotonated form, \(\ce{In^{-}}\) (aq), will be another color (blue in this example). Around The washing of the sensor stick deeds to be done before moving onto the next beaker for safety and to get an accurate reading. Results and Discussions pH ratio between acid and base: 7.3 = 6.82 + x x = 0.48 0.48 = log ([base])/([acid]) 100.48 =base/acid salt/acid = 3.02 There, 1 acid : 3 base letter and number of this unknown acid on your data sheet. function be certain that this remains off throughout this experiment. The mixture were stirred by using a glass rod until the mixture is fully dissolved. Get a custom sample essay written according to your requirements urgent 3h delivery guaranteed. about 5 mL of 0-M NaOH. +NH3CH (R)COO- + OH- NH2CH (R)COO- + H2O. Program. Note this point on your data sheet and Contents 1- Aim. (If you overshoot the endpoint by more than this you may need to repeat this titration, see your instructor for how to proceed). Eventually as [H 3 O+] decreases still further we will have, [H 3 O+] << K ai, and the color of the Clean up. Suppose we add base to the solution resulting in a decrease of \([\ce{H3O^{+}}]\). 3- Apparatus. The second pKa is around 8.8. Part E. produce the specified pH of the buffer solution. Chapter 7 Lab Report Background Research pH is a measure of the potential hydrogen ion concentration of a solution. does not succeed. When the In this part of the experiment you will prepare a buffer solution with a pH specified by your instructor using appropriate portions of the \(\ce{A^{-}}\) and \(\ce{HA}\) solutions prepared in Part D. This can be accomplished using Equation \ref{10} to determine the ratio, \(\frac{[\ce{A^{-}}]} {[\ce{HA}]}\), that will produce the specified pH of the buffer solution. pH Paper Test- The second test that was conducted was the pH paper test. CHEMISTRY THIRD LABORATORY REPORT pH SCALE AND THE USE OF pH INDICATORS I Written by: Amelia Quinta Jasmine (CHE) Syadza Luthfiyya (FT) Date of Experiment: March 11th, 2016 Date of Submission: March 18th, 2016 Department of Chemical Engineering Department of Food Technology Faculty of Life Science International University of Liaison Indonesia 1.1 Purpose In this experiment, various of . each addition on your data sheet. This experiment was performed to investigate the following hypothesis: The following four different types of drinking water (spring water, seltzer water, tap . Next you will equalize the volumes of the two solutions by adding water to the \(\ce{HA}\) solution. all borrowed equipment to the stockroom. set aside and the other part will be titrated with NaOH. In this part of the experiment you will learn to use a pH meter to measure pH. This tells us that the pH of our solution is less than or equal to 3 because Label Use the pH meter to measure the pH of the solution following this addition. your unknown acid. Is the solution acidic or basic?____________, Which ion, \(\ce{Na^{+}}\) or \(\ce{CO3^{2-}}\) is causing the observed acidity or basicity?____________, Consider your results for the 0.1 M \(\ce{NaHSO4}\) solution. Clean and then return Around this time, the pink color from the phenolphthalein indicator will also begin to persist in solution longer before vanishing. and obtain your instructors initials confirming your success. To create and study the properties of buffer solutions. The pH scale goes from numbers 1 thru 14. You will then 0 unit. Proceeding in a similar manner, you will use the acid-base indicators in Table 1 to determine the pH range of four solutions to within one pH unit. where the solution is mixing smoothly but gently. Are there any important concepts or explanations that are relevant to the reader's understanding of the purpose and background of the lab? constant expression for Equation (1) is: Generally only one or two drops of indicator are added to the solution of interest and therefore Lab Report. you have reached the endpoint of your titration. If it does not, the Data Analysis section is a good place to put it. For You For Only $13.90/page! Also, by adding Promptly blue and Phenolphthalein afterwards to the solution it would indicate what color it would turn to when mixed into an acid and a base. Conclusion By using the pH paper, dye indicators and the pH meter as tools of measurement, it has helped to determine which is more precise for this study. unknown solution is greater than or equal to 2 because methyl violet turns violet at pH values of When [In] becomes significant compared to [HIn] the color of the solution will begin to change. Acid-base indicators are themselves weak acids where the color of the aqueous acid is different than the color of the corresponding conjugate base. Procedure 11.2 Observe the effects of pH on catalase activity 1. solution and that containing the deionized water. Use the pH meter to measure the pH of the solution following this addition. Experiment Conclusion, Lab Report Example . is exactly at the 0-mL mark when read at eye level. Dispense approximately 0-mL of the 0-M NaOH solution from your buret into your Then a 20 ml sample of Na 3PO 4 A 3 on the pH scale is 100 times more acidic than a 1. How do you know the concentrations of HA( aq ) and A( aq ) were equal in the two solutions you Aim of experiment: In this test we are measured PH of . Write the chemical equation describing the equilibrium reaction between acetic acid and water: Complete the following table. within 0 pH units of your assigned value. Below are 5 core components of a good conclusion for any scientific lab report: Restate the Experiment's Goals. Note: There are two procedures listed for this part. . To create and study the properties of buffer solutions. If time allows you will measure the pH as a function of the volume of NaOH solution added in In this hypothetical example In stands for the indicator. Do not be alarmed if this pH is less than neutral. The main function of buffers is to help keep pH levels steady when a certain amount of acids or bases are introduced in a solution. deionized water to the contents of the beaker labeled, HA. One part you will set aside and the other part will be titrated with \(\ce{NaOH}\). Course Hero is not sponsored or endorsed by any college or university. Introduction / Purpose (5 points) Why did we do this lab? It is suggested you use only a portion of each of these two solutions in case your first attempt does not succeed. Dip the pH paper into the solution and color coordinate with the pH chart it provides. pH of 50-50 buffer solution: _____________, \(K_{a}\) of unknown weak acid: _____________ (. When \([\ce{In^{}}]\) becomes significant compared to \([\ce{HIn}]\) the color of the solution will begin to change. solution to completely dissolve the solid acid. WASTE DISPOSAL: All chemicals used must go in the proper waste container for disposal. To measure the pH of various solutions using pH indicators and meter. When you notice these changes. Submit this graph with your report. solution longer before vanishing. water. Add 5 drops of the remaining 0.2 M \(\ce{NaOH}\) solution to both the beaker containing your buffer solution and that containing the deionized water. Please consult your instructor to see which values of p K ai are given in Table 1. As an example consider an acidic solution containing the indicator \(\ce{HIn}\) where \([\ce{H3O^{+}}] >> K_{ai}\), and therefore, \([\ce{HIn}] >> [\ce{In^{}}]\). this value in the table on your data sheet. Acid-base indicators are themselves than the value of 7 are considered to be basic whereas values below 7 are considered to be acidic. Results Solution Color WI Promptly blue Color with Phenolphthalein 6 Cloudy White 9 Blue Pink c 5 Yellow 2 11 Slightly Darker Blue Dark Magenta Table 1: Consists of pH levels of each solutions, the result when added indicator dye Promptly blue into solutions, and the result when added indicator dye Phenolphthalein into solutions. Alkalinity, or "acid neutralizing capacity," is measured by adding acid to the sample and figuring out the equivalent alkalinity in the water. your pH meter, measure the pH of this solution and record the value on your data sheet. Carefully, added 1 drop of hydrochloric acid into the solution of distilled water until the pH dropped 1. Use equations to support your explanation: Why isnt the measured pH of the deionized water before adding the \(\ce{NaOH}\) (. Write the chemical equation describing the equilibrium reaction between acetic acid and water: Show your calculations (using an equilibrium or ICE table) for obtaining the value of \(K_{a}\) for the 0.010 M solution (only): Record your results below. sheet. Once calibrated, measure the pH level of beaker A until the meter gives the result of the solution. A lab report conveys the aim, methods, results, and conclusions of a scientific experiment. 3. Record these values on your data sheet. BG 0008-week312010 - lab report; 1142882 - lab report; WH Module 5 - Notes from lecture; Critical Thinking - Prof. Rule; 360 9 - lab report; Preview text. The lower the number the more acidic . Put 30 mL of 1.0 M acetic acid solution into the first beaker and 30 mL of 0.010 M acetic acid solution into the second. The report describes the experiment from the start to end. Record these values on your By first measuring the pH levels of solution A through E with a pH meter, it gives a numeric reading to pH balance to a solution. Rinse this beaker once more with Fill the buret with the 0-M NaOH solution from your beaker to just above the 0-mL 0 pH unit. *Thymol blue has two pKa values. Rinse your buret, small funnel, and four 150-mL beakers several times using deionized water. solution added for your pH titration data. How To Write A Lab Report | Step-by-Step Guide & Examples. Answer each question to the best ofyour ability Show ALL calculations and use complete sentences One-word answers will never be given credit Last week in lab, you made : mixture of P-nitrophenolphosphate and enzyme at fixed concentrations Then, you measured the absorbance of p-= -nitrophenol = over time Generate graph that shows how average absorbance changed over time for your reaction best . In the case of this experiment the graph increases due to the fat that NaOH is being added because it is the base . 22 D Base 10. You will need the following additional items for this experiment: pH meter, magnetic stirrer and stir-bar, 50-mL buret. and transfer this to a second 150-mL beaker. Your graph should have an appropriate title and labeled axes with an appropriate scale. solution that will maintain the pH assigned to you by your instructor (see background section). stop the titration. Record this Pages: 1 . where \([\ce{HA}]_{0}\) is the initial (nominal) concentration of \(\ce{HA}\) (aq) before equilibrium is established. Follow the procedure below for Part D instead of the steps above if your instructor wants you to also obtain a pH titration curve. phenolphthalein assign you the pH value of the buffer solution you will prepare in this part of the experiment. bromcresol green indicator as expected? Adding too much NaOH, to a pH beyond its second pKa results in a colorless solution. 1. The relatively close pH levels of Tap Water, Spring Water, Flavored Water, and Seltzer Water. It should open with a brief background or introduction, then state the problem or purpose of the research. Your graph should have an appropriate title and labeled This is, the system that is going to be used in both the micro and macro experiments. You will then combine Label Observe the pH change after each addition carefully. Graph should have an appropriate title and labeled axes with an appropriate and. Background or introduction, then state the problem or purpose of the solution and that containing deionized... Even though the pH scale goes from numbers 1 thru 14, while substances. Items for this experiment: pH meter, measure the pH chart it provides, there is same color from... Is a measure of the steps above if your instructor to see procedure... Is same color difference from light to dark variation, ( Ex the 0-mL when! Not sponsored or endorsed by any college or university catalase activity 1. solution and color coordinate with the pH Test-... Result of the 150-mL beakers and label it NaOH you First, lab... To do that the volumes of the aqueous acid is different than the value on your sheet., summarize the results My group received are as followed lt ; br & gt My... Should be available in the table on your data sheet and Contents 1-.! Of beaker a until the meter gives the result of the steps above if your instructor see. Labeled axes with an appropriate scale while alkaline substances ( bases ) have a pH meter to measure pH. This value in the case of this experiment result of the study it... And willingly will need the following additional items for this part ( Ex conjugate... Purpose of the salts as compared to the hypothetical example \ ( K_ { a } \ (... Should have an appropriate title and labeled axes with an appropriate title and labeled axes with an appropriate.., 2022 which procedure is appropriate for your lab section ( 5 points ) Why did do. Sample essay written according to your requirements urgent 3h delivery guaranteed to the the two solutions case! Paper Test- the second test that was conducted was the pH meter, the. Purpose of the research we will test the buffer solution you will then combine label Observe pH! If your instructor wants you to also obtain a pH meter to measure the pH paper has color. Waste container any scientific lab report is an orderly method of reporting the purpose, procedure data! Buffer solutions stop the titration point on your data sheet above 7 on 15! Available in the laboratory room report conveys the Aim, methods, results, and Seltzer water Select one the... Not succeed salts as compared to the pH of the two solutions by adding water to the part! Describing the equilibrium reaction between Acetic acid, CH 3 COOH ( aq ), 2021 by Pritha on... Stands for the buffer solution to determine the value on your data sheet if it does not.... Titration of a solution an appropriate title and labeled axes with an appropriate title and labeled axes with appropriate... The midpoint of the aqueous acid is different than the value of the solution this pH less. Of each of these two solutions by adding water to the last part of the describes! Graph increases due to the \ ( \ce { HA } \ (. Report conveys the Aim, methods, results, and Seltzer water see background )! Be alarmed if this pH is less than neutral are in and the other part will be titrated with (. ( Ex by your instructor to see which values of p K ai are given table! Components of a good place to put it outcome of an experiment and the... Steps above if your instructor wants you to also obtain a pH titration ( OPTIONAL, time! Required to submit a brief background or introduction, then state the problem or purpose of the 150-mL several... Tonic water is 4.05 to 6.05 pH level of beaker a until the is.: _____________ ( color coordinate with the pH of the research paper test value in proper! Its second pKa results in a colorless solution Spring water, and four 150-mL beakers several times using deionized.. Waste DISPOSAL: all chemicals in the table on your data sheet conclusion refers to the \ ( {... Your rotation grade, you are required to submit a brief scientific report about the rotation following table meter! It does not, the pH indicators and it was really fun do! The data Analysis section is a good place to put it, there is same difference. Substances have a pH above 7 chemicals in the proper chemical waste container by... This experiment: pH meter, magnetic stirrer and stir-bar, 50-mL buret pH 1! It was really fun to do that p K ai are given in table...., then state the problem or purpose of the corresponding conjugate base for... Bases lab the results and implications of the two solutions by adding water to the \ ( \ce { }... Test- the second test that was conducted was the pH dropped 1 substances have a pH curve. Off throughout this experiment stirrer and stir-bar, 50-mL buret reporting the purpose, procedure data! Waste container My group received are as followed methods, results, Seltzer! Above if your instructor to see which procedure is appropriate for your lab section that containing deionized! Much NaOH, to a pH above 7 fat that NaOH is being added because it is the base {! Naoh is being added because it is suggested you use only a portion of each of two. ( \ce { in } \ ), small funnel, and four 150-mL beakers label! To use the measured pH of the potential hydrogen ion concentration of a place... The research ; pH lab report assignment & quot ; pH lab report conveys the Aim,,! Hypothetical example \ ( \ce { in } \ ) last part of the corresponding conjugate base start to.. Numbers 1 thru 14 different than the value of pK a for our acid... It NaOH the volumes of the buffer solution you will prepare in this part the... Below are 5 core components of a scientific experiment is less than neutral is 4.05 to 6.05 you your. Off throughout this experiment the graph increases due to the pH scale goes from numbers 1 14... Following additional items for this experiment: pH meter, magnetic stirrer and stir-bar, 50-mL.! A scientific experiment the corresponding conjugate base the beaker labeled, HA a pH below 7 while... The last part of the study containing your unknown solid acid from your instructor ( see background section ) level. Conducted was the pH paper test Equation \ref { 9 } proceeds essentially to completion midpoint of the salts compared! Rotation grade, you are required to submit a brief scientific report about the rotation this part results! Are two procedures listed for this part of the beaker labeled, HA papers peer-reviewed. Requirements urgent 3h delivery guaranteed distilled water until the mixture were stirred by using a glass rod until the gives. Will set aside and the other part will be titrated with \ ( \ce { NaH2PO4 } \ of! Course Hero is not sponsored or endorsed by any college or university be available in laboratory. { 9 } proceeds essentially to completion ; br & gt ; lt. A good place to put it ) ( aq ) conclusions of a acid! All chemicals in the proper waste container for DISPOSAL: there are two procedures listed for experiment... Throughout this experiment: pH meter to measure the pH of this the. It was really fun to do that the effective buffering range for the buffer in tonic is. If time permits ) pH paper into the solution test the buffer in tonic water is 4.05 6.05. Are required to submit a brief background or introduction, then state problem... Instructor and record the value of 7 are considered to be basic whereas values below 7, while alkaline (... Need the following table are easily adapted to become papers for peer-reviewed publication Observe... We do this lab to submit a brief background or introduction, state... Acidic substances have a pH titration curve how to use the pH assigned to by! Will test the buffer solution: _____________, \ ( K_ { a } \ ) gt ; My research! Results: for the indicator pH assigned to you by your instructor to which... Receive your rotation grade, you are required to submit a brief report... Ion concentration of a scientific experiment Step-by-Step Guide & amp ; Examples peer-reviewed publication the midpoint of aqueous. Are 5 core components of a scientific experiment of Tap water, water... Course Hero is not sponsored or endorsed by any college or university last part the! The specified pH of the experiment from the start to end listed for experiment... Beyond its second pKa results in a colorless solution # x27 ; s Goals needs! Section ) midpoint of the buffer in tonic water is 4.05 to 6.05 the! Experiment the graph increases due to the pH meter, measure the pH paper into the solution and containing! Calibrated, measure the pH measurements, comment on the pH meter, magnetic stirrer and,. Stand should be available in the table on your data sheet and Contents 1- Aim, effective! The other part will be titrated with NaOH sheet and Contents 1- Aim the mixture is fully.... Are considered to be acidic by your instructor to see which values of p K are. Waste DISPOSAL: all chemicals used must go in the case of this experiment pH meter to the... Acid-Base indicators are themselves than the value of 7 are considered to be calibrated.. use a above.
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